Lipoprotein-associated phospholipase A(2), platelet-activating factor acetylhydrolase: a potential new risk factor for coronary artery disease

Atherosclerosis. 2000 Jun;150(2):413-9. doi: 10.1016/s0021-9150(99)00406-2.

Abstract

A specific and robust immunoassay for the lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), platelet-activating factor acetylhydrolase, is described for the first time. The immunoassay was used to evaluate possible links between plasma Lp-PLA(2) levels and atherosclerosis risk amongst susceptible individuals. Such an investigation was important because Lp-PLA(2) participates in the oxidative modification of low density lipoprotein by cleaving oxidised phosphatidylcholines, generating lysophosphatidylcholine and oxidised free fatty acids. The majority of Lp-PLA(2) was found associated with LDL (approximately 80%) and, as expected, enzyme levels were significantly positively correlated to LDL cholesterol. Plasma Lp-PLA(2) levels were significantly elevated in patients with angiographically proven coronary artery disease (CAD) when compared with age-matched controls, even though LDL cholesterol levels did not differ significantly. Indeed, when included in a general linear model with LDL cholesterol and other risk factors, Lp-PLA(2) appeared to be an independent predictor of disease status. We propose, therefore, that plasma Lp-PLA(2) mass should be viewed as a potential novel risk factor for CAD that provides information related to but additional to traditional lipoprotein measurements.

Publication types

  • Comparative Study

MeSH terms

  • 1-Alkyl-2-acetylglycerophosphocholine Esterase
  • Adult
  • Aged
  • Antibodies, Monoclonal
  • Arteriosclerosis / diagnostic imaging
  • Arteriosclerosis / enzymology*
  • Arteriosclerosis / etiology
  • Biomarkers / blood
  • Coronary Angiography
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Lipids / blood
  • Male
  • Middle Aged
  • Phospholipases A / blood*
  • Phospholipases A / immunology
  • Platelet Activating Factor / immunology
  • Platelet Activating Factor / metabolism*
  • Platelet Activation
  • Prognosis
  • Risk Factors

Substances

  • Antibodies, Monoclonal
  • Biomarkers
  • Lipids
  • Platelet Activating Factor
  • Phospholipases A
  • 1-Alkyl-2-acetylglycerophosphocholine Esterase