A monoclonal blocking EIA for herpes simplex virus type 2 antibody: validation for seroepidemiological studies in Africa

J Virol Methods. 2000 Jun;87(1-2):71-80. doi: 10.1016/s0166-0934(00)00149-x.


A competitive type-specific enzyme-linked immunosorbent assay (ELISA) for herpes simplex virus type 2 (HSV-2) antibody was developed using an infected cell antigen and a monoclonal antibody to glycoprotein G-2. This assay has been validated for use for epidemiological studies using a large panel of sera collected in rural Uganda and a panel of 143 sera characterised previously by Western blotting, the 'gold standard' for HSV type-specific serology. This evaluation was found to have a sensitivity of 96% and a specificity of 91% in comparison with Western blot on 143 sera from clinic patients. The ELISA had a sensitivity of 93% and a specificity of 91% in comparison with Western blot on 495 sera collected in Uganda. The assay showed good reproducibility and a low percentage of sera gave equivocal results, indicating its suitability for epidemiological studies.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal
  • Antibodies, Viral / blood*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Herpes Simplex / epidemiology*
  • Herpes Simplex / virology
  • Herpesvirus 2, Human / immunology
  • Herpesvirus 2, Human / isolation & purification*
  • Humans
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serologic Tests
  • Uganda / epidemiology


  • Antibodies, Monoclonal
  • Antibodies, Viral