Simple detection method for distinguishing dead and living yeast colonies

J Microbiol Methods. 2000 Jun;41(1):19-21. doi: 10.1016/s0167-7012(00)00136-6.


A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / cytology*
  • Basidiomycota / drug effects
  • Candida albicans / cytology*
  • Candida albicans / drug effects
  • Coloring Agents / toxicity
  • Gentian Violet / toxicity
  • Methylene Blue / toxicity
  • Rose Bengal / toxicity
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / drug effects
  • Schizosaccharomyces / cytology*
  • Schizosaccharomyces / drug effects
  • Trypan Blue / toxicity


  • Coloring Agents
  • Rose Bengal
  • Trypan Blue
  • Gentian Violet
  • Methylene Blue