Differential glycosylation of interleukin 2, the molecular basis for the NOD Idd3 type 1 diabetes gene?

Cytokine. 2000 May;12(5):477-82. doi: 10.1006/cyto.1999.0609.


The insulin-dependent diabetes (Idd) gene, Idd3, has been localised to a 0.35 cM region of chromosome 3 containing the structural gene for the cytokine interleukin 2 (IL-2). While variation of the N-terminal amino acid sequence of IL-2 has been shown to correlate with Idd3 allelic variation, differences in induction of proliferation by IL-2 allotypes have not been detected. In the current study, we examined the electrophoretic migration of IL-2 allotypes and have found two distinct patterns, consistent with differences in glycosylation, that correlate with diabetes-resistance and susceptibility. These findings strongly suggest that IL-2 variants may be functionally distinct.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Diabetes Mellitus, Type 1 / genetics*
  • Diabetes Mellitus, Type 1 / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Glycosylation
  • Interleukin-2 / genetics*
  • Interleukin-2 / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred NOD
  • Molecular Sequence Data


  • Interleukin-2