Polymorphism at NRAMP1 and D2S1471 loci associated with juvenile rheumatoid arthritis

Arthritis Rheum. 2000 Jun;43(6):1397-404. doi: 10.1002/1529-0131(200006)43:6<1397::AID-ANR25>3.0.CO;2-6.


Objective: To examine the role of NRAMP1 in susceptibility to juvenile rheumatoid arthritis (JRA).

Methods: DNA from 119 JRA patients (72 pauciarticular, 47 polyarticular) and 111 healthy controls from Latvia was genotyped for a functional repeat polymorphism in the promoter of NRAMP1 and a linked (<150 kb) microsatellite D2S1471. The findings were compared with those from HLA-DQ alleles typed previously. Chi-square analyses were performed using the Mantel-Haenszel test and stratification according to pure Latvian or pure Russian descent. Haplotype analysis was performed using the Associate program to implement the expectation-maximization algorithm based on the gene-counting technique.

Results: Allele 3 at NRAMP1 conferred increased risk (odds ratios [ORs] 2.26, 2.31, and 2.19; P = 0.0006, 0.003, and 0.019) of disease in the JRA, pauciarticular, and polyarticular patient groups, respectively. Allele 2 conferred protection (OR 0.44, 0.43, and 0.46). Alleles at D2S1471 that conferred susceptibility (6 and 12) or protection (11) did so only when on a haplotype with alleles 3 or 2, respectively, at NRAMP1. Allele 3 at NRAMP1 was additive with HLA-DQ7 for susceptibility (OR 3.71, 3.71, and 4.02), and allele 2 at NRAMP1 was additive with HLA-DQ5 for protection (OR 0.19, 0.08, and 0.12).

Conclusion: The NRAMP1 allele conferring susceptibility to JRA drives high levels of NRAMP1 expression, while the allele associated with protection drives low levels. These 2 alleles are inversely associated with susceptibility to infectious disease, consistent with their maintenance in populations through balancing selection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Arthritis, Juvenile / ethnology
  • Arthritis, Juvenile / genetics*
  • Arthritis, Juvenile / immunology
  • Carrier Proteins / genetics*
  • Cation Transport Proteins*
  • Child
  • Child, Preschool
  • Chromosome Mapping*
  • Genetic Predisposition to Disease*
  • HLA-DQ Antigens / analysis
  • Haplotypes
  • Humans
  • Infant
  • Latvia / ethnology
  • Membrane Proteins / genetics*
  • Microsatellite Repeats
  • Polymorphism, Genetic*
  • Russia / ethnology
  • White People / genetics*


  • Carrier Proteins
  • Cation Transport Proteins
  • HLA-DQ Antigens
  • HLA-DQ5 antigen
  • HLA-DQ7 antigen
  • Membrane Proteins
  • natural resistance-associated macrophage protein 1