Construction of a physical and transcript map flanking the imprinted MEST/PEG1 region at 7q32

Genomics. 2000 Jun 1;66(2):221-5. doi: 10.1006/geno.2000.6206.


MEST/PEG1, a gene expressed paternally in mesodermal derivatives in early embryonic stages, is the first imprinted gene mapped to chromosome 7q32. Since imprinted genes are clustered in general at a chromosomal region, we speculated that a similar imprinted-gene cluster may exist at chromosome region 7q32 and that the functions of some such genes may contribute to the phenotype of Silver-Russell syndrome including maternal uniparental disomy for chromosome 7 (maternal UPD7). As an initial step toward the isolation of imprinted genes at 7q32, we adopted an integrated approach involving the construction of a PAC contig and ESTs mapping in the vicinity of MEST. Here, we have constructed a complete contig of PAC and BAC clones and a transcript map spanning the entire approximately 1-Mb region between D7S530 and D7S649. We developed 60 novel STSs and precisely mapped 47 genes/ESTs. This map contains a putative autistic disorder locus that has been suggested to be localized near markers D7S530 and D7S684. This integrated physical and transcript map provides a valuable resource for identification of an imprinted gene(s) in this region as well as a candidate gene(s) for autistic disorder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Human, Pair 7*
  • DNA Primers
  • Genomic Imprinting*
  • Humans
  • Molecular Sequence Data
  • Physical Chromosome Mapping*
  • Proteins / genetics*
  • RNA, Messenger / genetics*


  • DNA Primers
  • Proteins
  • RNA, Messenger
  • mesoderm specific transcript protein

Associated data

  • GENBANK/AF208547
  • GENBANK/AF208548
  • GENBANK/AF208549
  • GENBANK/AF208550
  • GENBANK/AF208551
  • GENBANK/AF208552
  • GENBANK/AF208553
  • GENBANK/AF208554
  • GENBANK/AF208555
  • GENBANK/AF208556
  • GENBANK/AF208557
  • GENBANK/AF208558
  • GENBANK/AF208559
  • GENBANK/AF208560
  • GENBANK/AF208561
  • GENBANK/AF208562
  • GENBANK/AF208563
  • GENBANK/AF208564
  • GENBANK/AF208565
  • GENBANK/AF208566
  • GENBANK/AF208567
  • GENBANK/AF208568
  • GENBANK/AF208569
  • GENBANK/AF208570
  • GENBANK/AF208571
  • GENBANK/AF208572
  • GENBANK/AF208573
  • GENBANK/AF208574
  • GENBANK/AF208575