Ly-6I, a new member of the murine Ly-6 superfamily with a distinct pattern of expression

J Immunol. 2000 Jul 1;165(1):313-21. doi: 10.4049/jimmunol.165.1.313.


A new member of the mouse Ly-6SF, designated Ly-6I, has been isolated as a gene homologous to a segment of the Ly-6C gene. A single allelic difference in the mature protein sequence was identified, which is similar to other Ly-6SF members. Ly-6I mRNA has been detected in a wide range of tissues and cell lines, and a rabbit polyclonal Ab has been used to determine that Ly-6I protein is present at a low constitutive level on cell lines from several different lineages. In contrast to Ly-6C and Ly-6A/E, the Ly-6I gene is only weakly responsive to IFNs. Expression in vivo is most abundant on bone marrow populations and is coexpressed with Ly-6C on granulocytes and macrophages. However, Ly-6I is also expressed on immature B cell populations that do not express Ly-6C. Expression on mature B cells in spleen is uniformly low. Similarly, Ly-6I is expressed on TCRlow/int, but not TCRhigh, thymocytes. Ly-6I is re-expressed on Ly-6Chigh T cells in the periphery. Thus, Ly-6I may be a useful marker to define maturation stages of both T and B lymphocytes as well as subsets of monocytes and granulocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Antigens, Ly / biosynthesis
  • Antigens, Ly / genetics*
  • Antigens, Ly / isolation & purification*
  • Chromosomes / chemistry
  • Chromosomes / genetics
  • DNA, Complementary / chemistry
  • Gene Expression Regulation / immunology*
  • Granulocytes / immunology
  • Granulocytes / metabolism
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics*
  • Membrane Proteins / isolation & purification*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred NOD
  • Monocytes / immunology
  • Monocytes / metabolism
  • Multigene Family / immunology*
  • Tumor Cells, Cultured


  • Antigens, Ly
  • DNA, Complementary
  • Ly6a protein, mouse
  • Ly6i protein, mouse
  • Membrane Proteins