Expression, subcellular localization and putative function of platelet-type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential

Int J Cancer. 2000 Jul 1;87(1):37-43. doi: 10.1002/1097-0215(20000701)87:1<37::aid-ijc6>;2-l.


The involvement of 12-lipoxygenase (12-LOX) expression and function in tumor metastasis has been demonstrated in several murine tumor cell lines. In addition, 12-LOX expression was detected in human prostatic tumors and correlated to the clinical stage of disease. Here we provide data that human prostate cancer cell lines express the platelet-type isoform of 12-LOX at both the mRNA and protein levels, and immunohistochemistry revealed 12-LOX expression in human prostate tumors. The enzyme was localized to the plasma membrane, cytoplasmic organelles and nucleus in non-metastatic cells (PC-3 nm) and to the cytoskeleton and nucleus in metastatic cells (DU-145). After orthotopic/intraprostatic injection of tumor cells into SCID mice, the metastatic prostate carcinoma cells (DU-145) expressed 12-LOX at a significantly higher level compared with the non-metastatic counterparts, PC-3nm. The functional involvement of 12-LOX in the metastatic process was demonstrated when DU-145 cells were pretreated in vitro with the 12-LOX inhibitors N-benzyl-N-hydroxy-5-phenylpentamide (BHPP) or baicalein, the use of which significantly inhibited lung colonization. These data suggest a potential involvement of 12-LOX in the progression of human prostate cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arachidonate 12-Lipoxygenase / biosynthesis*
  • Arachidonate 12-Lipoxygenase / physiology*
  • Blood Platelets / enzymology*
  • Cell Membrane / enzymology
  • Cytoplasm / enzymology
  • Cytoskeleton / enzymology
  • Disease Progression
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Lipoxygenase Inhibitors
  • Male
  • Mice
  • Mice, SCID
  • Microscopy, Confocal
  • Neoplasm Invasiveness
  • Neoplasm Transplantation
  • Neoplasms, Experimental
  • Phenotype
  • Prostatic Neoplasms / enzymology*
  • Prostatic Neoplasms / pathology
  • Protein Isoforms
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured


  • Lipoxygenase Inhibitors
  • Protein Isoforms
  • RNA, Messenger
  • Arachidonate 12-Lipoxygenase