Expansions of clonal and oligoclonal T cells in B-cell chronic lymphocytic leukemia are primarily restricted to the CD3(+)CD8(+) T-cell population

Cytometry. 2000 Jun 15;42(3):188-95.


B-cell chronic lymphocytic leukemia (B-CLL) is characterized by the accumulation of mature-appearing clonal B cells exhibiting coexpression of CD5 and CD23. In addition to the accumulation of neoplastic B cells, numerous T-cell abnormalities also occur in B-CLL patients. In this study, the presence, and distribution within the T-cell subsets, of clonal/oligoclonal T cells was studied. Multicolor flow cytometric techniques were employed using combinations of anti-CD3, anti-CD4, and anti-CD8 antibodies coupled with antibodies specific for V(alpha) and V(beta) T-cell receptor (TCR) epitopes. Molecular studies of TCR gene sequences were done to confirm the presence of clonal/oligoclonal T-cell populations. In the flow cytometric studies, examination of V(alpha)/V(beta)expression found evidence of clonal/oligoclonal expansion in 9 of 19 patients studied. In eight of the nine patients, the expansions were restricted to the CD3(+)CD8(+) cell population. Molecular analyses were performed in 16 patients, 12 of whom showed a clonal or oligoclonal pattern. Of the four patients who were negative in the molecular analyses, all demonstrated flow cytometric evidence of clonal/oligoclonal expansions. Thus, when the flow cytometric and molecular analyses were considered together, all 16 patients for whom parallel analyses were done showed evidence of clonal/oligoclonal expansions. These results confirm previous work demonstrating that the majority of B-CLL patients harbor clonal/oligoclonal expansions within the T-cell population. Additionally, based on the relative numbers of cells expressing specific V(alpha) or V(beta)epitopes, these results show that these expansions occur primarily within the CD3(+)CD8(+) T-cell population.

MeSH terms

  • Aged
  • Aged, 80 and over
  • CD3 Complex / analysis*
  • CD4 Antigens / analysis
  • CD8 Antigens / analysis*
  • Clone Cells / immunology
  • Female
  • Flow Cytometry
  • Gene Rearrangement
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / blood*
  • Lymphocyte Count
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell, gamma-delta / genetics
  • T-Lymphocytes / immunology*


  • CD3 Complex
  • CD4 Antigens
  • CD8 Antigens
  • Receptors, Antigen, T-Cell, gamma-delta