Rapid and reliable protein extraction from yeast

Yeast. 2000 Jun 30;16(9):857-60. doi: 10.1002/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B.


The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / analysis
  • Fungal Proteins / isolation & purification*
  • Hydrogen-Ion Concentration
  • Mycology / methods
  • Pichia / chemistry*
  • Pichia / growth & development
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae / growth & development


  • Fungal Proteins