The discovery of new highly efficient tetra repeat STR loci, development of fluorescence multicolour dye technology and capillary electrophoresis have made it possible to amplify ten loci in a single reaction. This combination provides an extraordinary effectiveness of simultaneous amplification and detection. With this method it became possible to determine individual identity and paternity at an enhanced level of precision and accuracy in 1 to 2 days with a high biostatistical probability. This review demonstrates the role of automated fluorescent multicolour dye genotyping technology in forensic paternity testing.