Antibody to B. subtilis DNA polymerase III: use in enzyme purification and examination of homology among replication-specific DNA polymerases

Nucleic Acids Res. 1979 Mar;6(3):1203-19. doi: 10.1093/nar/6.3.1203.


Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B. subtilis pol III.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies*
  • Antigen-Antibody Reactions
  • Bacillus subtilis / enzymology*
  • DNA Polymerase III / immunology
  • DNA Polymerase III / isolation & purification*
  • DNA Replication*
  • DNA-Directed DNA Polymerase / isolation & purification*
  • Kinetics
  • Molecular Weight
  • Rabbits / immunology
  • Species Specificity


  • Antibodies
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase