Rearrangement Generated in Double Genes, NSP1 and NSP3, of Viable Progenies From a Human Rotavirus Strain

Virus Res. 2000 Apr;67(2):163-71. doi: 10.1016/s0168-1702(00)00139-8.


We generated rotavirus clones with rearrangement in vitro by serial passages of a human rotavirus strain (IGV-80-3) at high multiplicity of infection and determined nucleotide sequences of the rearranged genes from two distinct rotavirus clones, each of which possesses two rearranged genes: a common rearranged NSP1 gene and NSP3 gene with slightly different migration in polyacrylamide gel electrophoresis. Sequence analysis showed that the rearranged NSP1 and NSP3 genes had similar gene structures: concatemerization in a head to tail orientation and partial duplication of the open reading frame following the termination codon. The rearranged NSP1 gene had a direct repeat, whereas in the rearranged NSP3 gene, no such pattern was found.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cloning, Organism
  • Gene Rearrangement
  • Genes, Viral*
  • Genetic Variation
  • Humans
  • Molecular Sequence Data
  • RNA, Viral / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / genetics*
  • Sequence Analysis


  • RNA, Viral

Associated data

  • GENBANK/AF190169
  • GENBANK/AF190170
  • GENBANK/AF190171
  • GENBANK/AF190172
  • GENBANK/X59297