The role of the bacitracin ABC transporter in bacitracin resistance and collateral detergent sensitivity

FEMS Microbiol Lett. 2000 Jul 1;188(1):103-6. doi: 10.1111/j.1574-6968.2000.tb09176.x.

Abstract

The bacitracin resistance of Bacillus licheniformis, a producer of bacitracin, is mediated by the ABC transporter Bcr. Bacillus subtilis cells carrying bcr genes on high-copy number plasmids developed collateral detergent sensitivity, as did human cells with overexpressed multidrug resistance P-glycoprotein. Resistance against bacitracin and sensitivity of resistant cells to detergents were shown to be inseparable phenomena associated with the membrane part of Bcr transporter, namely protein BcrC. A fused protein, consisting of ATP-binding protein BcrA and membrane component BcrC was constructed. It resembled a half molecule of P-glycoprotein and was capable of providing a significant degree of antibiotic resistance and detergent sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism*
  • Bacillus / drug effects*
  • Bacillus / genetics
  • Bacillus / metabolism
  • Bacillus subtilis / drug effects
  • Bacillus subtilis / genetics
  • Bacillus subtilis / growth & development
  • Bacitracin / metabolism
  • Bacitracin / pharmacology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Detergents / pharmacology*
  • Drug Resistance, Microbial
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Deletion
  • Plasmids / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Transformation, Bacterial

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • Detergents
  • Recombinant Fusion Proteins
  • bacitracin permease
  • Bacitracin