Aquaporin 3 Cloned From Xenopus Laevis Is Regulated by the Cystic Fibrosis Transmembrane Conductance Regulator

FEBS Lett. 2000 Jun 23;475(3):291-5. doi: 10.1016/s0014-5793(00)01689-6.

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is essential for epithelial electrolyte transport and has been shown to be a regulator of epithelial Na(+), K(+), and Cl(-) channels. CFTR also enhances osmotic water permeability when activated by cAMP. This was detected initially in Xenopus oocytes and is also present in human airway epithelial cells, however, the mechanisms remain obscure. Here, we show that CFTR activates aquaporin 3 expressed endogenously and exogenously in oocytes of Xenopus laevis. The interaction requires stimulation of wild type CFTR by cAMP and an intact first nucleotide binding domain as demonstrated for other CFTR-protein interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aquaporin 3
  • Aquaporins / genetics*
  • Aquaporins / metabolism*
  • Cloning, Molecular
  • Cyclic AMP / metabolism
  • Cystic Fibrosis Transmembrane Conductance Regulator / metabolism*
  • Female
  • Humans
  • Molecular Sequence Data
  • Oocytes
  • Xenopus laevis

Substances

  • Aquaporins
  • CFTR protein, human
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Aquaporin 3
  • Cyclic AMP