Abstract
We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular
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Fungal Proteins / genetics
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Genes, Fungal*
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Genes, Lethal
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Genetic Complementation Test
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Meiosis / genetics*
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Molecular Sequence Data
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Mutation
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Phenotype
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RNA Precursors / genetics
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RNA Splicing / genetics*
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Ribonucleoprotein, U4-U6 Small Nuclear
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Ribonucleoprotein, U5 Small Nuclear
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S Phase / genetics
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Saccharomyces / genetics*
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces pombe Proteins*
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Sequence Alignment
Substances
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Fungal Proteins
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PRP6 protein, S cerevisiae
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RNA Precursors
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Ribonucleoprotein, U4-U6 Small Nuclear
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Ribonucleoprotein, U5 Small Nuclear
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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prp31 protein, S pombe