De novo design and structural characterization of proteins and metalloproteins

Annu Rev Biochem. 1999;68:779-819. doi: 10.1146/annurev.biochem.68.1.779.


De novo protein design has recently emerged as an attractive approach for studying the structure and function of proteins. This approach critically tests our understanding of the principles of protein folding; only in de novo design must one truly confront the issue of how to specify a protein's fold and function. If we truly understand proteins, it should be possible to design receptors, enzymes, and ion channels from scratch. Further, as this understanding evolves and is further refined, it should be possible to design proteins and biomimetic polymers with properties unprecedented in nature.

Publication types

  • Review

MeSH terms

  • Metalloproteins / chemistry*
  • Protein Folding
  • Protein Structure, Secondary
  • Proteins / chemistry*


  • Metalloproteins
  • Proteins