Molecular Cloning of a Cyclin-Like Protein Associated With Cyclin-Dependent Kinase 3 (Cdk 3) in Vivo

Biochem Biophys Res Commun. 2000 Jul 5;273(2):442-7. doi: 10.1006/bbrc.2000.2965.

Abstract

cdk3 has been considered to be rate-limiting for cell cycle progression of mammalian cells while its precise function remains to be elucidated. To assess cdk3 function, a cDNA coding for a cyclin-like protein (designated as ik3-1 from an interactor-1 with cdk3) was isolated with the yeast two-hybrid system using a cyclin-dependent kinase 3 (cdk3) cDNA as bait. p70(ik3-1) (a 70-kDa protein designated as p70(ik3-1)) seems to belong to the cyclin family as its C-terminal domain composed of 124 amino acids resembles the highly conserved cyclin box. Coimmunoprecipitation indicated that p70(ik3-1) binds to p35(cdk3) in vivo. The ik3-1 gene may belong to a multigene family and is highly conserved during evolution. mRNA expression of ik3-1 was low in the early G1 phase, upregulated during G1 progression, maximal at a mid-late G1 point, and declined gradually thereafter, suggesting that it may work mainly in G1 phase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • COS Cells
  • Carrier Proteins*
  • Cloning, Molecular
  • Cyclin-Dependent Kinase 3
  • Cyclin-Dependent Kinases / metabolism*
  • Cyclins / chemistry
  • Cyclins / genetics*
  • Cyclins / metabolism
  • DNA, Complementary / genetics
  • G1 Phase
  • Gene Expression
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • Phosphoproteins*
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Two-Hybrid System Techniques

Substances

  • CABLES1 protein, human
  • Cables1 protein, mouse
  • Carrier Proteins
  • Cyclins
  • DNA, Complementary
  • Phosphoproteins
  • RNA, Messenger
  • Cdk3 protein, mouse
  • Cyclin-Dependent Kinase 3
  • Cyclin-Dependent Kinases