Phylogenetic Classification of Prokaryotic and Eukaryotic Sir2-like Proteins

Biochem Biophys Res Commun. 2000 Jul 5;273(2):793-8. doi: 10.1006/bbrc.2000.3000.

Abstract

Sirtuins (Sir2-like proteins) are present in prokaryotes and eukaryotes. Here, two new human sirtuins (SIRT6 and SIRT7) are found to be similar to a particular subset of insect, nematode, plant, and protozoan sirtuins. Molecular phylogenetic analysis of 60 sirtuin conserved core domain sequences from a diverse array of organisms (including archaeans, bacteria, yeasts, plants, protozoans, and metazoans) shows that eukaryotic Sir2-like proteins group into four main branches designated here as classes I-IV. Prokaryotic sirtuins include members of classes II and III. A fifth class of sirtuin is present in gram positive bacteria and Thermotoga maritima. Saccharomyces cerevisiae has five class I sirtuins. Caenorhabditis elegans and Drosophila melanogaster have sirtuin genes from classes I, II, and IV. The seven human sirtuin genes include all four classes: SIRT1, SIRT2, and SIRT3 are class I, SIRT4 is class II, SIRT5 is class III, and SIRT6 and SIRT7 are class IV.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Conserved Sequence
  • DNA, Complementary / genetics
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics
  • Eukaryotic Cells
  • Evolution, Molecular
  • Histone Deacetylases / chemistry
  • Histone Deacetylases / classification*
  • Histone Deacetylases / genetics*
  • Humans
  • Models, Genetic
  • Molecular Sequence Data
  • Phylogeny
  • Prokaryotic Cells
  • Protein Structure, Tertiary / genetics
  • Sequence Homology, Amino Acid
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae*
  • Sirtuin 1
  • Sirtuin 2
  • Sirtuins
  • Trans-Activators / chemistry
  • Trans-Activators / classification*
  • Trans-Activators / genetics*

Substances

  • DNA, Complementary
  • Drosophila Proteins
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae
  • Trans-Activators
  • SIR2 protein, S cerevisiae
  • SIRT1 protein, human
  • Sirt2 protein, Drosophila
  • Sirtuin 1
  • Sirtuin 2
  • Sirtuins
  • Histone Deacetylases