Phenytoin and its metabolite, 5-(4-hydroxyphenyl)-5-phenylhydantoin, show bone resorption in cultured neonatal mouse calvaria

Jpn J Pharmacol. 2000 Jan;82(1):82-4. doi: 10.1254/jjp.82.82.


The effects of phenytoin and its major metabolite, 5-(4-hydroxyphenyl)-5-phenylhydantoin (HPPH), on bone resorption of neonatal mouse calvaria were examined in vitro. Both phenytoin and HPPH induced significant bone resorption as compared to the controls after 72 h in culture. This effect may be the cause of phenytoin-induced bone loss in vivo.

MeSH terms

  • Animals
  • Bone Resorption / chemically induced*
  • Mice
  • Organ Culture Techniques
  • Phenytoin / analogs & derivatives*
  • Phenytoin / metabolism
  • Phenytoin / toxicity*
  • Skull / drug effects


  • hydroxyphenytoin
  • Phenytoin