Analysis of period circadian expression in the Drosophila head by in situ hybridization

J Neurogenet. 1997 Nov;11(3-4):255-63. doi: 10.3109/01677069709115099.

Abstract

The expression of the period (per) gene of Drosophila melanogaster has been studied by in situ hybridization in the adult's head, where it is required for the fly to exhibit behavioral circadian rhythms. We have used non-radioactive in situ hybridization to obtain a high sensitivity and specificity on head sections, with single cell resolution. Consistent with previous per protein- or per reporter gene-expression, per-expressing cells were detected in the optic lobes and the central brain, as well as in the head sensory organs: eyes, ocelli, maxillary palps and proboscis. In the brain and the eyes, circadian fluctuations of the per mRNA abundance were observed in different per expressing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Circadian Rhythm / physiology*
  • Drosophila Proteins
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / physiology
  • Head
  • In Situ Hybridization
  • Nuclear Proteins / genetics*
  • Period Circadian Proteins

Substances

  • Drosophila Proteins
  • Nuclear Proteins
  • PER protein, Drosophila
  • Period Circadian Proteins