Cutting edge: Chandra, a novel four-transmembrane domain protein differentially expressed in helper type 1 lymphocytes

J Immunol. 2000 Jul 15;165(2):632-6. doi: 10.4049/jimmunol.165.2.632.


Development of naive Th cells into Th1 and Th2 effector populations requires coordinated expression of a complex set of genes. In this study, we have identified a novel four-transmembrane domain protein, Chandra, that is differentially expressed in Th1 cells. Chandra expression is observed in STAT4- and IFN-gamma-deficient mice, indicating that Chandra is not an IL-12- or IFN-gamma-responsive gene. Interestingly, Chandra mRNA is detected in anti-CD3-activated T cells from STAT6-deficient mice in the absence of any differentiation conditions. Furthermore, neutralization of IL-4 signaling is sufficient to induce transcription of Chandra in anti-CD3-activated T cells from wild-type mice, demonstrating that STAT6 signaling is required to repress Chandra expression in activated T cells and Th2 subsets. This is the first demonstration of a differentially expressed four-transmembrane domain protein in Th1 cells.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • DNA-Binding Proteins / physiology
  • Female
  • Gene Expression Regulation / immunology
  • Interleukin-4 / physiology
  • Membrane Proteins / antagonists & inhibitors
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • STAT1 Transcription Factor
  • STAT4 Transcription Factor
  • Signal Transduction / immunology
  • Th1 Cells / metabolism*
  • Trans-Activators / physiology


  • DNA-Binding Proteins
  • Membrane Proteins
  • Ms4a4b protein, mouse
  • STAT1 Transcription Factor
  • STAT4 Transcription Factor
  • Stat1 protein, mouse
  • Stat4 protein, mouse
  • Trans-Activators
  • Interleukin-4