Abstract
A human pancreatic cancer cell line, Capan-1, secretes the chemokines interleukin-8 (IL-8) and growth-related oncogene alpha (GROalpha). Capan-1 cells also express the chemokine receptor 2 (CXCR2), which is a Gialpha-protein coupled receptor. Growth of Capan-1 cells was inhibited when anti-IL-8 or anti-GROalpha monoclonal antibody was added into the culture medium. Pertussis toxin, which blocks Gialpha also demonstrated a growth-inhibitory effect on Capan-1 cells. These results indicated that IL-8 and GROalpha act on Capan-1 cells as growth factors in an autocrine manner through CXCR2.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal / pharmacology
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Cell Division* / drug effects
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Chemokine CXCL1
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Chemokines, CXC*
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Chemotactic Factors / genetics
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Chemotactic Factors / physiology*
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Growth Inhibitors / genetics
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Growth Inhibitors / physiology*
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Growth Substances / genetics
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Growth Substances / physiology*
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Humans
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Intercellular Signaling Peptides and Proteins*
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Interleukin-8 / genetics
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Interleukin-8 / physiology*
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Pancreatic Neoplasms
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Pertussis Toxin
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Receptors, Interleukin-8B / genetics
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Transcription, Genetic*
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Tumor Cells, Cultured
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Virulence Factors, Bordetella / pharmacology
Substances
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Antibodies, Monoclonal
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CXCL1 protein, human
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Chemokine CXCL1
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Chemokines, CXC
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Chemotactic Factors
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Growth Inhibitors
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-8
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Receptors, Interleukin-8B
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Virulence Factors, Bordetella
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Pertussis Toxin