Autocrine growth effect of IL-8 and GROalpha on a human pancreatic cancer cell line, Capan-1

Pancreas. 2000 Jul;21(1):52-6. doi: 10.1097/00006676-200007000-00051.


A human pancreatic cancer cell line, Capan-1, secretes the chemokines interleukin-8 (IL-8) and growth-related oncogene alpha (GROalpha). Capan-1 cells also express the chemokine receptor 2 (CXCR2), which is a Gialpha-protein coupled receptor. Growth of Capan-1 cells was inhibited when anti-IL-8 or anti-GROalpha monoclonal antibody was added into the culture medium. Pertussis toxin, which blocks Gialpha also demonstrated a growth-inhibitory effect on Capan-1 cells. These results indicated that IL-8 and GROalpha act on Capan-1 cells as growth factors in an autocrine manner through CXCR2.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / pharmacology
  • Cell Division* / drug effects
  • Chemokine CXCL1
  • Chemokines, CXC*
  • Chemotactic Factors / genetics
  • Chemotactic Factors / physiology*
  • Growth Inhibitors / genetics
  • Growth Inhibitors / physiology*
  • Growth Substances / genetics
  • Growth Substances / physiology*
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-8 / genetics
  • Interleukin-8 / physiology*
  • Pancreatic Neoplasms
  • Pertussis Toxin
  • Receptors, Interleukin-8B / genetics
  • Transcription, Genetic*
  • Tumor Cells, Cultured
  • Virulence Factors, Bordetella / pharmacology


  • Antibodies, Monoclonal
  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokines, CXC
  • Chemotactic Factors
  • Growth Inhibitors
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Receptors, Interleukin-8B
  • Virulence Factors, Bordetella
  • Pertussis Toxin