The FBP interacting repressor targets TFIIH to inhibit activated transcription

Mol Cell. 2000 Feb;5(2):331-41. doi: 10.1016/s1097-2765(00)80428-1.


FUSE-binding protein (FBP) binds the single-stranded far upstream element of active c-myc genes, possesses potent transcription activation and repression domains, and is necessary for c-myc expression. A novel 60 kDa protein, the FBP interacting repressor (FIR), blocked activator-dependent, but not basal, transcription through TFIIH. Recruited through FBP's nucleic acid-binding domain, FIR formed a ternary complex with FBP and FUSE. FIR repressed a c-myc reporter via the FUSE. The amino terminus of FIR contained an activator-selective repression domain capable of acting in cis or even in trans in vivo and in vitro. The repression domain of FIR targeted only TFIIH's p89/XPB helicase, required at several stages in transcription, but not factors required for promoter selection. Thus, FIR locks TFIIH in an activation-resistant configuration that still supports basal transcription.

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • DNA Helicases / antagonists & inhibitors
  • DNA-Binding Proteins / antagonists & inhibitors
  • DNA-Binding Proteins / metabolism*
  • HeLa Cells
  • Humans
  • Macromolecular Substances
  • Models, Genetic
  • Molecular Sequence Data
  • Peptide Fragments / metabolism
  • Protein Binding
  • Proto-Oncogene Proteins c-myc / genetics*
  • RNA Splicing Factors
  • RNA-Binding Proteins
  • Regulatory Sequences, Nucleic Acid
  • Repressor Proteins / metabolism*
  • Transcription Factor TFIIH
  • Transcription Factors / metabolism*
  • Transcription Factors, TFII*
  • Transcriptional Activation*
  • Two-Hybrid System Techniques


  • DNA-Binding Proteins
  • FUBP1 protein, human
  • Macromolecular Substances
  • Peptide Fragments
  • Proto-Oncogene Proteins c-myc
  • RNA Splicing Factors
  • RNA-Binding Proteins
  • Repressor Proteins
  • Transcription Factors
  • Transcription Factors, TFII
  • poly-U binding splicing factor 60KDa
  • XPBC-ERCC-3 protein
  • Transcription Factor TFIIH
  • DNA Helicases

Associated data

  • GENBANK/AF190744