The JAK/STAT signaling pathway is required for the initial choice of sexual identity in Drosophila melanogaster

Mol Cell. 2000 Mar;5(3):581-7. doi: 10.1016/s1097-2765(00)80451-7.

Abstract

The choice of sexual identity in Drosophila is determined by a system that measures the X chromosome to autosome ratio (X/A). This system depends upon unequal expression of X-linked numerator genes in 1X and 2X nuclei. The numerators activate a special Sxl promoter, Sxl-Pe, in 2X/2A nuclei, but not 1X/2A nuclei. By multimerizing a conserved Sxl-Pe sequence block, we generated a gain-of-function promoter, Sxl-PeGOF, that is inappropriately active in 1X/2A nuclei. GOF activity requires the X-linked unpaired (upd) gene, which encodes a ligand for the Drosophila JAK/STAT signaling pathway. upd also functions as a numerator element in regulating wild-type Sxl-Pe reporters. We demonstrate that the JAK kinase, Hopscotch, and the STAT DNA-binding protein, Marelle, are also required for Sxl-Pe activation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Conserved Sequence
  • DNA-Binding Proteins / metabolism*
  • Drosophila Proteins*
  • Drosophila melanogaster / embryology*
  • Drosophila melanogaster / genetics
  • Female
  • Glycoproteins / metabolism
  • JNK Mitogen-Activated Protein Kinases
  • Janus Kinases
  • Mitogen-Activated Protein Kinases / metabolism
  • Promoter Regions, Genetic
  • Protein Kinases / metabolism*
  • Protein-Tyrosine Kinases / metabolism
  • RNA-Binding Proteins / metabolism
  • Repetitive Sequences, Amino Acid
  • Sex Determination Processes*
  • Signal Transduction
  • Trans-Activators / metabolism*
  • Transcription Factors*

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • Glycoproteins
  • RNA-Binding Proteins
  • Sxl protein, Drosophila
  • Trans-Activators
  • Transcription Factors
  • upd1 protein, Drosophila
  • Protein Kinases
  • Protein-Tyrosine Kinases
  • Janus Kinases
  • hop protein, Drosophila
  • JNK Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinases