Structural characterization of the cysteine-rich domain of TFIIH p44 subunit

J Biol Chem. 2000 Oct 13;275(41):31963-71. doi: 10.1074/jbc.M004960200.


In an effort to understand the structure function relationship of TFIIH, a transcription/repair factor, we focused our attention on the p44 subunit, which plays a central role in both mechanisms. The amino-terminal portion of p44 has been shown to be involved in the regulation of the XPD helicase activity; here we show that its carboxyl-terminal domain is essential for TFIIH transcription activity and that it binds three zinc atoms through two independent modules. The first contains a C4 zinc finger motif, whereas the second is characterized by a CX(2)CX(2-4)FCADCD motif, corresponding to interleaved zinc binding sites. The solution structure of this second module reveals an unexpected homology with the regulatory domain of protein kinase C and provides a framework to study its role at the molecular level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Cysteine* / genetics
  • Cysteine* / metabolism
  • DNA Helicases / chemistry
  • DNA Helicases / genetics
  • DNA Helicases / metabolism
  • Histidine / genetics
  • Histidine / metabolism
  • Humans
  • Mass Spectrometry
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Binding
  • Protein Kinase C / chemistry
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Protein Subunits
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Structure-Activity Relationship
  • Transcription Factor TFIIH
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription Factors, TFII*
  • Transcription, Genetic
  • Zinc / metabolism
  • Zinc Fingers*


  • Protein Subunits
  • Recombinant Proteins
  • Transcription Factors
  • Transcription Factors, TFII
  • Transcription Factor TFIIH
  • Histidine
  • Protein Kinase C
  • DNA Helicases
  • Zinc
  • Cysteine

Associated data

  • PDB/1E53