Effects of selected herbicides on cytokine production in vitro

Life Sci. 2000 May 19;66(26):2519-25. doi: 10.1016/s0024-3205(00)00586-5.


To evaluate possible deleterious effects of commonly used herbicides on leukocytes, cytokine production was selected as a sensitive indicator. After in vitro exposure of human peripheral blood mononuclear cells from normal donors, the production of all 3 cytokines tested--interferon-gamma (a type 1 cytokine), interleukin-5 (a type 2 cytokine) and tumor necrosis factor-alpha (an inflammatory cytokine)--was impaired by up to 70, 50 and 70% respectively in a concentration-dependent manner in cultures exposed to atrazine (0.03-3 microM in 1% dimethylsulfoxide, DMSO). The effect paralleled that seen with dexamethasone, a known immunosuppressive agent. Other pesticides also dissolved in DMSO--mecoprop, simazine or MCPA (each up to 1 microM)--or dissolved in phosphate-buffered saline--diuron (up to 1 microM), isoproturon (up to 3 microM), metoxuron (up to 8 microM) or metamitron (up to 80 microM)--showed no concentration-related effects on cytokine production. There was, however, an inhibition of IFN-gamma and TNF-alpha production by simazine, metoxuron and mecoprop and of all three cytokines tested by diuron. MCPA (0.01 and 0.1 microM) stimulated the production of TNF-alpha. Thus, exposure to herbicides leading to plasma levels in the micromolar range induces imbalance in cytokine production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media, Serum-Free
  • Cytokines / biosynthesis*
  • Enzyme-Linked Immunosorbent Assay
  • Herbicides / pharmacology*
  • Humans
  • In Vitro Techniques
  • Interferon-gamma / biosynthesis
  • Interleukin-5 / biosynthesis
  • Leukocytes / drug effects*
  • Leukocytes / metabolism
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Culture Media, Serum-Free
  • Cytokines
  • Herbicides
  • Interleukin-5
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma