Regulation of expression of nuclear factor kappa B RelA in oligodendrocytes: effect of hypoxia

Neuroreport. 2000 Jun 26;11(9):1877-81. doi: 10.1097/00001756-200006260-00015.

Abstract

Little is known about the expression of nuclear factor kappa B (NF-kappaB) in oligodendrocytes. We demonstrate that under normal culture conditions only 21% of oligodendrocytes express NF-kappaB p65; exposure to hypoxia increased this to 69%. A similar increase in total p65 was seen with Western blots. Northern blot analysis demonstrated that NF-kappaB p65 mRNA increased during the first few h following hypoxia, suggesting that exposure to hypoxia affects NF-kappaB p65 transcription or p65 mRNA stability. Under normoxic conditions only 2% of the oligodendrocytes exhibited activated NF-kappaB; this was increased to 8% by hypoxia followed by reoxygenation. Despite activation of NF-kappaB, no detectable expression of the pro-inflammatory genes iNOS and ICAM-1 was seen. This is the first demonstration that hypoxia can up-regulate p65.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Cells, Cultured
  • Hypoxia / metabolism*
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1 / metabolism
  • NF-kappa B / genetics
  • NF-kappa B / metabolism*
  • Nitric Oxide Synthase / metabolism
  • Nitric Oxide Synthase Type II
  • Oligodendroglia / metabolism*
  • RNA, Messenger / metabolism
  • Rats
  • Reference Values
  • Transcription Factor RelA

Substances

  • NF-kappa B
  • RNA, Messenger
  • Transcription Factor RelA
  • Intercellular Adhesion Molecule-1
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, rat