Sequence-based analysis of mutagenized mice

Mamm Genome. 2000 Jul;11(7):594-7. doi: 10.1007/s003350010113.

Abstract

Treating mice with ethylnitrosourea (ENU) is an efficient means for mutagenizing spermatogonial cells, and this treatment has been proven effective in a variety of screens for both dominant and recessive mutations. However, a significant problem for this technology is that the efficiency of mutagenesis is assessed most often by the empiric determination of a per-locus mutation frequency by using the specific locus test, which is expensive, time-consuming, and logistically difficult. To approach this question more directly and more efficiently, one can utilize methods of PCR-based mutation detection for the characterization of progeny of mutagenized mice. Since this analysis can be done after a single generation of breeding, it is useful as a rapid means for the assessment of the efficiency of mutagen treatment. Furthermore, it is readily imaginable that this strategy can be applied for the general determination of gene function in a systematic manner. Theoretical considerations and empirical analysis suggest that the per-base mutation frequency for a fractionated-dose treatment protocol is on the order of 1 sequence change per 10(5) bp.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • DNA Mutational Analysis*
  • Ethylnitrosourea
  • Genetic Carrier Screening
  • Genetic Testing
  • Mice / genetics*
  • Mutagenesis
  • Mutagens
  • Polymorphism, Single-Stranded Conformational

Substances

  • Mutagens
  • Ethylnitrosourea