Chronic metabolic acidosis increases NaDC-1 mRNA and protein abundance in rat kidney

Kidney Int. 2000 Jul;58(1):206-15. doi: 10.1046/j.1523-1755.2000.00155.x.


Background: Chronic metabolic acidosis increases, while alkali feeding inhibits, proximal tubule citrate absorption. The activity of the apical membrane Na+/citrate cotransporter is increased in metabolic acidosis, but is not altered by alkali feeding.

Methods: Renal cortical mRNA and brush border membrane protein abundances of sodium/dicarboxylate-1 (NaDC-1), the apical membrane Na+/citrate transporter, were measured.

Results: By immunohistochemistry, NaDC-1 was localized to the apical membrane of the proximal tubule. Chronic metabolic acidosis caused an increase in NaDC-1 protein abundance that was maximal in the S2 segment and that increased with time. Metabolic acidosis also increased NaDC-1 mRNA abundance, but this was first seen at three hours and correlated with the severity of the metabolic acidosis. Alkali feeding had no effect on NaDC-1 protein or mRNA abundance.

Conclusions: Chronic metabolic acidosis increases renal cortical NaDC-1 mRNA abundance and apical membrane NaDC-1 protein abundance, while alkali feeding is without effect on NaDC-1.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acidosis / chemically induced
  • Acidosis / metabolism*
  • Acids / pharmacology
  • Acute Disease
  • Alkalies / pharmacology
  • Ammonium Chloride / pharmacology
  • Animals
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Chronic Disease
  • Citrates / urine
  • Dicarboxylic Acid Transporters*
  • Gene Expression / physiology
  • Kidney Cortex / metabolism
  • Kidney Tubules, Proximal / metabolism*
  • Male
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Organic Anion Transporters, Sodium-Dependent*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Bicarbonate / pharmacology
  • Symporters*


  • Acids
  • Alkalies
  • Carrier Proteins
  • Citrates
  • Dicarboxylic Acid Transporters
  • Membrane Proteins
  • Organic Anion Transporters, Sodium-Dependent
  • RNA, Messenger
  • SLC13A2 protein, human
  • Slc13a2 protein, rat
  • Symporters
  • Ammonium Chloride
  • Sodium Bicarbonate