Interleukin-8 levels and activity in delayed-healing human thermal wounds

Wound Repair Regen. May-Jun 2000;8(3):216-25. doi: 10.1046/j.1524-475x.2000.00216.x.

Abstract

There are numerous causes for slow or delayed wound healing. Because slowly healing wounds are often inflamed, we quantitated the inflammatory chemokine, interleukin-8, produced by slowly healing human burn wounds and compared this to interleukin-8 from healed wounds and normal intact skin. Interleukin-8 levels were increased significantly in unhealed wounds (19.7 ng/ml) compared to healed wounds (7.7 ng/ml) or normal skin (5.7 ng/ml). Interleukin-8 in these ranges was added to adult human keratinocytes and fibroblasts. Interleukin-8 significantly decreased keratinocyte replication but had no effect on fibroblast replication. The rate or final degree of fibroblast populated collagen lattice contraction was inhibited at interleukin-8 concentrations between 10 and 30 ng/ml, but not altered at concentrations below 10 ng/ml and above 100 ng/ml. The concurrent application of indomethacin at 10 microg/ml reversed this interleukin-8 induced inhibition. Interleukin-8 inhibited myosin ATPase activity, apparently by reducing the phosphorylation of nonmuscle myosin light chain. We conclude that elevated levels of interleukin-8 may be found during delayed healing, and these elevated interleukin-8 levels may directly contribute to retarded wound closure.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Burns / physiopathology*
  • Enzyme-Linked Immunosorbent Assay
  • Epidermal Cells
  • Humans
  • Indomethacin / pharmacology
  • Interleukin-8 / analysis*
  • Keratinocytes
  • Myosin Light Chains
  • Phosphorylation
  • Time Factors
  • Wound Healing / physiology*

Substances

  • Interleukin-8
  • Myosin Light Chains
  • Indomethacin