Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa

Genes Dev. 2000 Jul 1;14(13):1589-94.


The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polkappa), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polkappa bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polkappa in the mutagenic bypass of certain types of DNA lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Base Sequence
  • DNA Damage / genetics*
  • DNA, Bacterial
  • Escherichia coli / enzymology
  • Escherichia coli Proteins*
  • Humans


  • Bacterial Proteins
  • DNA, Bacterial
  • DinB protein, E coli
  • Escherichia coli Proteins