CCR8 on human thymocytes functions as a human immunodeficiency virus type 1 coreceptor

J Virol. 2000 Aug;74(15):6946-52. doi: 10.1128/jvi.74.15.6946-6952.2000.


To determine whether human immunodeficiency virus type 1 (HIV-1) coreceptors besides CXCR4 and CCR5 are involved in HIV-1 infection of the thymus, we focused on CCR8, a receptor for the chemokine I-309, because of its high expression in the thymus. Similar levels of CCR8 mRNA were detected in immature and mature primary human thymocytes. Consistent with this, [(125)I]I-309 was shown to bind specifically and with similar affinity to the surface of immature and mature human thymocytes. Fusion of human thymocytes with cells expressing HIV-1 X4 or X4R5 envelope glycoprotein was inhibited by I-309 in a dose-dependent manner. In addition, I-309 partially inhibited productive infection of human thymocytes by X4, R5, and X4R5 HIV-1 strains. Our data provide the first evidence that CCR8 functions as an HIV-1 coreceptor on primary human cells and suggest that CCR8 may contribute to HIV-1-induced thymic pathogenesis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chemokine CCL1
  • Chemokine CXCL12
  • Chemokines
  • Chemokines, CC / metabolism
  • Chemokines, CC / pharmacology
  • Chemokines, CXC / pharmacology
  • Child, Preschool
  • Down-Regulation
  • Gene Products, env / metabolism
  • HIV Infections / virology*
  • HIV-1 / metabolism*
  • HIV-1 / pathogenicity
  • Humans
  • Infant
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, CCR8
  • Receptors, CXCR4 / metabolism
  • Receptors, Chemokine / genetics
  • Receptors, Chemokine / metabolism*
  • Receptors, HIV / metabolism*
  • T-Lymphocytes / metabolism
  • T-Lymphocytes / virology*


  • CCL1 protein, human
  • CCR8 protein, human
  • CXCL12 protein, human
  • Chemokine CCL1
  • Chemokine CXCL12
  • Chemokines
  • Chemokines, CC
  • Chemokines, CXC
  • Gene Products, env
  • RNA, Messenger
  • Receptors, CCR8
  • Receptors, CXCR4
  • Receptors, Chemokine
  • Receptors, HIV