Topical treatment with nerve growth factor for neurotrophic keratitis

Ophthalmology. 2000 Jul;107(7):1347-51; discussion 1351-2. doi: 10.1016/s0161-6420(00)00163-9.


Objective: To evaluate the efficacy of nerve growth factor (NGF) in patients with neurotrophic keratitis.

Design: Prospective, noncomparative, interventional case series.

Participants: Forty-five eyes of 43 consecutive patients with moderate (stage 2, n = 17) to severe (stage 3, n = 28) neurotrophic keratitis unresponsive to other nonsurgical therapies.

Methods: After a 10-day washout with preservative-free artificial tears, 45 eyes with neurotrophic keratitis received murine NGF (200 microg/ml) every 2 hours for 2 days followed by one drop six times daily until the ulcer healed. A maintenance dose of one drop NGF (100 microg/ml) was administered four times daily for the 2 weeks subsequent to ulcer healing.

Main outcome measures: Size and depth of the ulcer or the epithelial defect, corneal sensitivity, best corrected visual acuity, side effects, and relapse of the disease in the follow-up period.

Results: All patients had a complete resolution of the persistent epithelial defect (with or without an ulcer) after 12 days to 6 weeks of treatment with NGF. Patients affected by both stages of the disease demonstrated both improved corneal sensitivity and visual acuity (P<0.001). No significant differences were observed in the time to complete corneal healing between stage 2 and stage 3 patients. Hyperemia and ocular and periocular pain were side effects reported during the first days of treatment. No relapse of the disease was observed during the follow-up period, with the exception of three patients with trigeminal nerve resection, who required a single retreatment.

Conclusions: Nerve growth factor eye drops improved corneal sensitivity and promoted corneal epithelial healing in both moderate and severe neurotrophic keratitis. Although performed in an uncontrolled and nonrandomized series of patients, this therapy shows promise for the restoration of ocular surface integrity and visual function in neurotrophic corneal disease.

Publication types

  • Clinical Trial

MeSH terms

  • Administration, Topical
  • Adolescent
  • Adult
  • Aged
  • Child
  • Cornea / innervation*
  • Cornea / pathology
  • Cranial Nerve Diseases / drug therapy*
  • Cranial Nerve Diseases / pathology
  • Drug Evaluation
  • Female
  • Humans
  • Keratitis / drug therapy*
  • Keratitis / pathology
  • Male
  • Middle Aged
  • Nerve Growth Factor / administration & dosage
  • Nerve Growth Factor / therapeutic use*
  • Ophthalmic Nerve / drug effects*
  • Ophthalmic Nerve / pathology
  • Ophthalmic Solutions / administration & dosage
  • Ophthalmic Solutions / therapeutic use
  • Prospective Studies
  • Sensation
  • Treatment Outcome


  • Ophthalmic Solutions
  • Nerve Growth Factor