Genetic regulation: the Lac control region

Science. 1975 Jan 10;187(4171):27-35. doi: 10.1126/science.1088926.

Abstract

The nucleotide sequence of the lac promoter-operator region has been determined. The 122 base pairs comprising this region include the recognition sites for RNA polymerase, the positive regulatory protein, CAP, and the negative regulatory protein, the repressor. Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Chromosome Mapping
  • Codon
  • Computers
  • DNA, Bacterial / analysis
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / metabolism*
  • Galactosidases / biosynthesis
  • Genes, Regulator
  • Hydrolysis
  • Lactose / metabolism*
  • Models, Biological
  • Mutation
  • Nucleic Acid Hybridization
  • Oligonucleotides / analysis
  • Operon*
  • Phosphorus Radioisotopes
  • RNA, Bacterial
  • RNA, Messenger
  • Ribonucleases
  • Transcription, Genetic

Substances

  • Codon
  • DNA, Bacterial
  • Oligonucleotides
  • Phosphorus Radioisotopes
  • RNA, Bacterial
  • RNA, Messenger
  • DNA-Directed RNA Polymerases
  • Ribonucleases
  • Galactosidases
  • Lactose

Associated data

  • GENBANK/J01636
  • GENBANK/J01637
  • GENBANK/K01483
  • GENBANK/K01793