Optimized synthesis of RNA-protein fusions for in vitro protein selection

Methods Enzymol. 2000;318:268-93. doi: 10.1016/s0076-6879(00)18058-9.
No abstract available

MeSH terms

  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • Codon
  • DNA / metabolism
  • DNA, Complementary / metabolism
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Gene Library
  • Genes, myc / genetics
  • Genetic Techniques
  • Magnesium / chemistry
  • Models, Genetic
  • Oligonucleotides / chemistry
  • Peptides / chemistry
  • Phosphorylation
  • Polymerase Chain Reaction
  • Potassium / chemistry
  • Protein Biosynthesis
  • Protein Processing, Post-Translational
  • Proteins / chemistry
  • Proteins / metabolism*
  • Puromycin / chemistry
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism*
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics
  • Reticulocytes / metabolism
  • Ribosomes / chemistry

Substances

  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • Codon
  • DNA, Complementary
  • Oligonucleotides
  • Peptides
  • Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Puromycin
  • DNA
  • Magnesium
  • Potassium