Inhibition of azoxymethane-induced colon cancer by orange juice

Nutr Cancer. 2000;36(2):224-9. doi: 10.1207/S15327914NC3602_12.


Previous research has shown that hesperidin, a flavanone glycoside in orange juice, inhibits colon carcinogenesis and that feeding double-strength orange juice delays the onset of chemically induced mammary cancer in rats. This study determined whether feeding single-strength, pasteurized orange juice would inhibit azoxymethane (AOM)-induced colon cancer in male Fischer 344 rats. Colon cancer was initiated by injecting AOM (15 mg/kg body wt) at 22 and 29 days of age. One week after the second AOM injection, orange juice replaced drinking water for the experimental group (n = 30). The rats were killed 28 weeks later, and tumors were removed for histological analysis. Feeding orange juice reduced tumor incidence by 22% (p < 0.05). Tumor reduction was associated with a decreased labeling index and proliferation zone in the colonic mucosa. Hesperidin, other flavonoids, limonin 17-beta-D-glucopyranoside, and other limonoid glucosides are potential chemopreventive agents in orange juice that could account for the decreased colon tumorigenesis associated with feeding orange juice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / therapeutic use*
  • Azoxymethane
  • Beverages*
  • Carcinogens
  • Citrus / chemistry
  • Citrus / therapeutic use*
  • Colon / physiopathology*
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / prevention & control*
  • Male
  • Phytotherapy*
  • Rats
  • Rats, Inbred F344


  • Antineoplastic Agents
  • Carcinogens
  • Azoxymethane