Mass spectrometry: a tool for the identification of proteins separated by gels

Electrophoresis. 2000 Jun;21(11):2105-14. doi: 10.1002/1522-2683(20000601)21:11<2105::AID-ELPS2105>3.0.CO;2-M.


Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high-resolution two-dimensional (2-D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments during the last five years in MS associated with protein gel separation are reviewed.

Publication types

  • Review

MeSH terms

  • Automation
  • Database Management Systems
  • Electrophoresis, Gel, Two-Dimensional
  • Mass Spectrometry / methods*
  • Peptide Mapping
  • Proteins / chemistry*
  • Proteins / isolation & purification


  • Proteins