Retinoic Acid Early Inducible Genes Define a Ligand Family for the Activating NKG2D Receptor in Mice

Immunity. 2000 Jun;12(6):721-7. doi: 10.1016/s1074-7613(00)80222-8.

Abstract

Here we describe a family of GPI-anchored cell surface proteins that function as ligands for the mouse activating NKG2D receptor. These molecules are encoded by the retinoic acid early inducible (RAE-1) and H60 minor histocompatibility antigen genes on mouse chromosome 10 and show weak homology with MHC class I. Expression of the NKG2D ligands is low or absent on normal, adult tissues; however, they are constitutively expressed on some tumors and upregulated by retinoic acid. Ectopic expression of RAE-1 and H60 confers target susceptibility to NK cell attack. These studies identify a family of ligands for the activating NKG2D receptor on NK and T cells, which may play an important role in innate and adaptive immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carcinoma, Lewis Lung
  • Cloning, Molecular / methods
  • Cytotoxicity, Immunologic / drug effects
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology*
  • Glycosylphosphatidylinositols / metabolism
  • Humans
  • Immunoglobulin Fc Fragments / genetics
  • Immunoglobulin G / genetics
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism*
  • Ligands
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Membrane Proteins / physiology
  • Mice
  • Mice, Inbred C57BL
  • Minor Histocompatibility Antigens / biosynthesis
  • Minor Histocompatibility Antigens / genetics
  • Minor Histocompatibility Antigens / metabolism
  • Minor Histocompatibility Antigens / physiology
  • Molecular Sequence Data
  • Multigene Family / immunology*
  • NK Cell Lectin-Like Receptor Subfamily K
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism*
  • Receptors, Natural Killer Cell
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Tretinoin / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Glycosylphosphatidylinositols
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • KLRK1 protein, human
  • Klrk1 protein, mouse
  • Ligands
  • Membrane Proteins
  • Minor Histocompatibility Antigens
  • NK Cell Lectin-Like Receptor Subfamily K
  • Raet1a protein, mouse
  • Raet1d, protein, mouse
  • Receptors, Immunologic
  • Receptors, Natural Killer Cell
  • Recombinant Fusion Proteins
  • minor H antigen H60
  • Tretinoin

Associated data

  • GENBANK/AF257520