Abstract
Biosynthesis of many classes of secondary metabolites in plants is induced by the stress hormone jasmonate. The gene for ORCA3, a jasmonate-responsive APETALA2 (AP2)-domain transcription factor from Catharanthus roseus, was isolated by transferred DNA activation tagging. Orca3 overexpression resulted in enhanced expression of several metabolite biosynthetic genes and, consequently, in increased accumulation of terpenoid indole alkaloids. Regulation of metabolite biosynthetic genes by jasmonate-responsive AP2-domain transcription factors may link plant stress responses to changes in metabolism.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acetates / pharmacology
-
Cell Line
-
Cyclopentanes / pharmacology
-
DNA, Bacterial
-
Gene Expression Regulation, Plant*
-
Homeodomain Proteins / chemistry
-
Magnoliopsida / genetics*
-
Molecular Sequence Data
-
Nuclear Proteins / chemistry
-
Oxylipins
-
Plant Growth Regulators / pharmacology
-
Plant Proteins / chemistry
-
Plant Proteins / genetics*
-
Plant Proteins / physiology
-
Transcription Factors / chemistry
-
Transcription Factors / genetics*
-
Transcription Factors / physiology
-
Vinca Alkaloids / biosynthesis
-
Vinca Alkaloids / metabolism
Substances
-
Acetates
-
Cyclopentanes
-
DNA, Bacterial
-
Homeodomain Proteins
-
Nuclear Proteins
-
ORCA3 protein, plant
-
Oxylipins
-
Plant Growth Regulators
-
Plant Proteins
-
T-DNA
-
Transcription Factors
-
Vinca Alkaloids
-
methyl jasmonate
Associated data
-
GENBANK/AJ251249
-
GENBANK/AJ251250