Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Jul;61(1):37-42.
doi: 10.1159/000028378.

M(2) and M(4) Muscarinic Receptor Subtypes Couple to Activation of Endothelial Nitric Oxide Synthase

Affiliations

M(2) and M(4) Muscarinic Receptor Subtypes Couple to Activation of Endothelial Nitric Oxide Synthase

D K Waid et al. Pharmacology. .

Abstract

In this report we studied coupling of M(2) and M(4) muscarinic acetylcholine receptors to activation of endothelial nitric oxide synthase (eNOS). Chinese hamster ovary cells that co-express the individual receptor subtypes and eNOS in a stable fashion were used as a model. Activation of eNOS was assayed by measuring increasing levels of cyclic GMP in admixed cells that contain guanylate cyclase. Activation of both M(2) or M(4) muscarinic receptors resulted in marked activation of eNOS, in a time- and concentration-dependent manner. The time course of the response exhibited a transient peak, followed by a sustained lower plateau. While the sustained phase was dependent on influx of extracellular calcium, the transient response showed dependency on both mobilization of intracellular calcium and extracellular influx.

Similar articles

See all similar articles

Cited by 5 articles

Publication types

LinkOut - more resources

Feedback