Up-regulation of gut-enriched krüppel-like factor by interferon-gamma in human colon carcinoma cells

FEBS Lett. 2000 Jul 14;477(1-2):67-72. doi: 10.1016/s0014-5793(00)01764-6.

Abstract

Interferon-gamma (IFN-gamma) induces growth arrest and apoptosis of tumor cells but the mechanisms for these functions are unknown. Recently, gut-enriched krüppel-like factor (GKLF) was found to possess similar biological properties. Treatment of HT-29 cells with IFN-gamma inhibited cell proliferation and induced apoptosis, the effect was found to associate with GKLF expression. IFN-gamma stimulates GKLF mRNA and protein levels in a dose- and time-dependent manner and this process is independent of p53, occurs rapidly and does not require de novo protein synthesis indicating that GKLF is an immediate-early IFN-gamma-responsive gene. Moreover, overexpression of GKLF results in similar effect as IFN-gamma suggesting that GKLF may function as a downstream target of IFN-gamma.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Blotting, Western
  • Cell Division / drug effects
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / metabolism*
  • Colonic Neoplasms / pathology
  • DNA-Binding Proteins*
  • Dactinomycin / pharmacology
  • Dose-Response Relationship, Drug
  • Humans
  • Interferon-gamma / pharmacology*
  • Kinetics
  • Kruppel-Like Transcription Factors
  • Protein Biosynthesis / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Substrate Specificity
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*
  • Transfection
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / physiology
  • Up-Regulation / drug effects*

Substances

  • Antineoplastic Agents
  • DNA-Binding Proteins
  • GKLF protein
  • Kruppel-Like Transcription Factors
  • RNA, Messenger
  • Transcription Factors
  • Tumor Suppressor Protein p53
  • Dactinomycin
  • Interferon-gamma