Immunohistochemical detection of membrane-type-1-matrix metalloproteinase in colorectal carcinoma

Br J Cancer. 2000 Jul;83(2):215-8. doi: 10.1054/bjoc.2000.1195.


We investigated whether the expression of membrane-type-1 matrix metalloproteinase (MT1-MMP), matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) was consistent with the proposed roles of these proteins in promoting metastasis in colorectal cancer. The expression of MT1-MMP was significantly more frequent in deeply invasive carcinomas (P = 0.007) and in cases of vascular invasion (P = 0.02). The frequency of detection of MMP-2 in the stroma was much greater in vascular invasion-positive cases (42%) than in negative cases (20%; P = 0.02). The rate of detection of TIMP-2 in tumour cell cytoplasm increased with the depth of invasion (P = 0.03). TIMP-2 in the stroma was found more frequently in tumours with lymphatic invasion and lymph node metastasis (P < 0.05). Significant correlations were found between detection of MT1-MMP and MMP-2 in tumour cell cytoplasm (P < 0.05), of MT1-MMP and TIMP-2 in tumour cell cytoplasm (P < 0.01), and of MMP-2 and TIMP-2 in tumour cell cytoplasm (P < 0.01). Immunohistochemical detection of MT1-MMP and TIMP-2 might be useful for monitoring infiltration in colorectal carcinoma but is not correlated with distant metastases.

MeSH terms

  • Adenocarcinoma / chemistry
  • Adenocarcinoma / enzymology*
  • Adenocarcinoma / pathology
  • Colorectal Neoplasms / chemistry
  • Colorectal Neoplasms / enzymology*
  • Colorectal Neoplasms / pathology
  • Humans
  • Immunohistochemistry
  • Matrix Metalloproteinase 2 / analysis
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases / analysis*
  • Tissue Inhibitor of Metalloproteinase-2 / analysis


  • Tissue Inhibitor of Metalloproteinase-2
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases
  • Matrix Metalloproteinase 2