Arabidopsis thaliana: a source of candidate disease-resistance genes for Brassica napus

Genome. 2000 Jun;43(3):452-60. doi: 10.1139/g00-008.


Common structural and amino acid motifs among cloned plant disease-resistance genes (R genes), have made it possible to identify putative disease-resistance sequences based on DNA sequence identity. Mapping of such R-gene homologues will identify candidate disease-resistance loci to expedite map-based cloning strategies in complex crop genomes. Arabidopsis thaliana expressed sequence tags (ESTs) with homology to cloned plant R genes (R-ESTs), were mapped in both A. thaliana and Brassica napus to identify candidate R-gene loci and investigate intergenomic collinearity. Brassica R-gene homologous sequences were also mapped in B. napus. In total, 103 R-EST loci and 36 Brassica R-gene homologous loci were positioned on the N-fo-61-9 B. napus genetic map, and 48 R-EST loci positioned on the Columbia x Landsberg A. thaliana map. The mapped loci identified collinear regions between Arabidopsis and Brassica which had been observed in previous comparative mapping studies; the detection of syntenic genomic regions indicated that there was no apparent rapid divergence of the identified genomic regions housing the R-EST loci.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Blotting, Southern
  • Brassica / genetics*
  • Chromosome Mapping*
  • DNA, Plant / analysis
  • Expressed Sequence Tags
  • Plant Diseases / genetics*
  • Polymorphism, Restriction Fragment Length
  • Sequence Homology, Nucleic Acid


  • DNA, Plant