SLO-2, a K+ channel with an unusual Cl- dependence

Nat Neurosci. 2000 Aug;3(8):771-9. doi: 10.1038/77670.


The gating of different potassium channels depends on many diverse factors. We now report a unique example of a K+ channel with a Cl - dependence. The slo-2 gene was cloned from Caenorhabditis elegans and is widely expressed in both neurons and muscles; it was highly abundant, as suggested by its high representation in the C. elegans EST database. SLO-2, like its paralogue, SLO-1, was also dependent on Ca 2+. We show by site-directed mutagenesis that its requirements for both Cl- and Ca2+ are synergistic and associated with the same functional domain. SLO-2's dependence on Cl - implies that intracellular Cl- homeostasis may be important in regulating cellular excitability through this unusual K+ channel.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins*
  • Calcium / pharmacology
  • Chlorides / pharmacology
  • Chlorides / physiology*
  • Conserved Sequence
  • Green Fluorescent Proteins
  • Helminth Proteins / physiology
  • Large-Conductance Calcium-Activated Potassium Channels
  • Luminescent Proteins / genetics
  • Mammals
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Muscles / physiology
  • Neurons / physiology*
  • Potassium Channels / chemistry
  • Potassium Channels / genetics
  • Potassium Channels / physiology*
  • Potassium Channels, Calcium-Activated*
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • Caenorhabditis elegans Proteins
  • Chlorides
  • Helminth Proteins
  • Large-Conductance Calcium-Activated Potassium Channels
  • Luminescent Proteins
  • Membrane Transport Proteins
  • Potassium Channels
  • Potassium Channels, Calcium-Activated
  • SLO-2 protein, C elegans
  • Green Fluorescent Proteins
  • Calcium