Cloning of the full-length coding sequence of rat liver-specific organic anion transporter-1 (rlst-1) and a splice variant and partial characterization of the rat lst-1 gene

Biochem Biophys Res Commun. 2000 Jul 21;274(1):79-86. doi: 10.1006/bbrc.2000.3105.


The full-length coding sequence of rat liver-specific organic anion transporter-1 (lst-1) and its splice variant have been cloned. The full-length rat lst-1 (designated rlst-1a) encodes a protein containing 687 amino acids and has 12-putative transmembrane domains, multiple potential N-glycosylation and phosphorylation sites. Therefore, rat lst-1a has 35 additional amino acid residues compared to the previously reported rat lst-1. A splice variant (designated rlst-1c) reported in this communication encodes a protein containing 654 amino acids and has 10-putative transmembrane domains. PCR analysis suggests that rlst-1a is the most abundant form in liver. Phylogenetic analysis reveals that rat lst-1a is an ortholog of human LST-1 (hLST-1) and mouse lst-1 (mlst-1). The rlst-1 gene is composed of 15 exons and 14 introns. Analysis of exon-intron boundary reveals that the splice variant rlst-1c lacks the entire exon 7, while the previously reported rat lst-1 (designated herein as rlst-1b) lacks approximately half of exon 10, and the splicing has occurred through alternative usage of a splice donor site within exon 10.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Anion Transport Proteins
  • Blotting, Northern
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • Codon
  • DNA, Complementary / metabolism
  • Exons
  • Humans
  • Introns
  • Liver / metabolism
  • Membrane Proteins / chemistry
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Protein Isoforms
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • Anion Transport Proteins
  • Carrier Proteins
  • Codon
  • DNA, Complementary
  • Membrane Proteins
  • Protein Isoforms

Associated data

  • GENBANK/AF208545
  • GENBANK/AF217450
  • GENBANK/AF272557
  • GENBANK/AF272571