Regulation of IL-1beta-mediated MUC2 gene in NCI-H292 human airway epithelial cells

Biochem Biophys Res Commun. 2000 Jul 21;274(1):112-6. doi: 10.1006/bbrc.2000.3107.


This study demonstrates for the first time the effects of IL-1beta on the regulation of protein production as well as MUC2 gene transcription in cultured human airway epithelial cells. The effect of IL-1beta on the regulation of MUC2 protein was determined by flow cytometric analysis. The expression level of MUC2 induced by IL-1beta increased in a dose-dependent manner. MUC2 transcripts were detected after 2 h of exposure to IL-1beta and reached maximal level after 8 h. Actinomycin D experiments indicated that the IL-1beta-mediated MUC2 expression was controlled by transcriptional regulation. Both RT-PCR and FACS analysis showed that budesonide concomitantly attenuated IL-1beta mediated MUC2 gene as well as protein production levels. Use of the glucocorticoid receptor antagonist, RU-486, restored the inhibitory effect of budesonide on the IL-1beta-mediated MUC2 protein as well as gene. The data suggest that IL-1beta up-regulates MUC2 gene by transcriptional regulation and that budesonide suppresses the IL-1beta-medicated MUC2 expression via decreased transcriptional activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bronchodilator Agents / pharmacology
  • Budesonide / pharmacology
  • Cell Separation
  • Dactinomycin / pharmacology
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Gene Expression Regulation* / drug effects
  • Hormone Antagonists / pharmacology
  • Humans
  • Interleukin-1 / metabolism*
  • Lung / cytology*
  • Mifepristone / pharmacology
  • Mucin-2
  • Mucins / genetics
  • Mucins / metabolism*
  • Nucleic Acid Synthesis Inhibitors
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Transcription, Genetic / drug effects
  • Transcriptional Activation
  • Tumor Cells, Cultured
  • Up-Regulation


  • Bronchodilator Agents
  • Hormone Antagonists
  • Interleukin-1
  • MUC2 protein, human
  • Mucin-2
  • Mucins
  • Nucleic Acid Synthesis Inhibitors
  • Dactinomycin
  • Mifepristone
  • Budesonide