Effect of omega 3 fatty acids on oxidative stress in humans: GC-MS measurement of urinary F2-isoprostane excretion

Redox Rep. 2000;5(1):45-6. doi: 10.1179/rer.2000.5.1.45.


Despite the reported benefits associated with omega3 fatty acids for cardiovascular disease, there remains concern that increased intake may lead to increased lipid peroxidation. To date, however, the data, particularly in vivo, are inconclusive. This report describes two interventions, one providing daily fish meals and the other eicosapentaenoic acid (EPA, 20:5 omega3) or docosahexaenoic acid (DHA, 22:6 omega3), the two principal omega3 fatty acids in marine oils, in which in vivo lipid peroxidation was assessed by measurement of urinary excretion of F2-isoprostanes. In both trials, urinary F2-isoprostanes were significantly reduced by 20-27%. Therefore, in contrast with previous reports in the literature, these results demonstrate that omega3 fatty acids reduce in vivo oxidant stress in humans.

Publication types

  • Clinical Trial
  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Diabetes Mellitus, Type 2 / urine
  • Dinoprost / analogs & derivatives*
  • Dinoprost / urine*
  • F2-Isoprostanes
  • Fatty Acids, Omega-3 / administration & dosage*
  • Fatty Acids, Omega-3 / urine
  • Fish Oils / administration & dosage
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Male
  • Oxidative Stress / drug effects*


  • F2-Isoprostanes
  • Fatty Acids, Omega-3
  • Fish Oils
  • 8-epi-prostaglandin F2alpha
  • Dinoprost