Abstract
Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Arginine / metabolism
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Biological Transport, Active
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Carbon Radioisotopes
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Culture Media
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Escherichia coli / isolation & purification*
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Escherichia coli / metabolism
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Isoleucine / metabolism
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Mutagens
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Mutation*
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Nitrosoguanidines
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Penicillins
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Proline / metabolism
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Temperature
Substances
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Carbon Radioisotopes
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Culture Media
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Mutagens
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Nitrosoguanidines
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Penicillins
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Isoleucine
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Arginine
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Proline